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Cell counting technology plays an important role in measuring the cell density in a culture tank. Cell culture is a process in which cells grow under control. This paper focuses on the cell detection and segmentation an efficient method is proposed for automatically detecting the cells with fluorescence pattern in an IIF image.
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How to segment the cells from an IIF image is essential in developing an automatic inspection system for ANA testing. Automatic inspection for fluorescence patterns in an IIF image can assist physicians, without relevant experience, in making correct diagnosis. The ANA testing allows us to scan a broad range of autoantibody entities and to describe them by distinct fluorescence patterns. PMID:29201677Īn Automatic Indirect Immunofluorescence Cell Segmentation Systemĭirectory of Open Access Journals (Sweden)įull Text Available Indirect immunofluorescence (IIF with HEp-2 cells has been used for the detection of antinuclear autoantibodies (ANA in systemic autoimmune diseases. Euroasian J Hepato-Gastroenterol 2015 5(1):7-10. Hepatitis B Core Antigen in Hepatocytes of Chronic Hepatitis B: Comparison between Indirect Immunofluorescence and Immunoperoxidase Method. How to cite this article Raihan R, Tabassum S, Al-Mahtab M, Nessa A, Jahan M, Kabir CMS, Kamal M, Aguilar JC. This seems to reflect the magnitudes of HBV replication in CHB. Conclusion Hepatitis B core antigen can be detected using the IIF from formalin fixed paraffin block preparation and also by IIP method. This was more evident in the case of HBeAg negative patients and showed a positive relation with HBV DNA levels. A positive relation among viral load and HBcAg detection was also found. Out of 62 patients with HBeAg-negative CHB, HBcAg was detected by IIF in 55 (88.7%) patients and by IIP in 51 (82.26%) patients.
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Results All HBeAg positive patients expressed HBcAg by both IIF and IIP methods. Hepatitis B core antigen was detected by indirect immunofluorescence (IIF) and indirect immunoperoxidase (IIP) methods in liver tissue. Out of 70 patients, eight (11.4%) were hepatitis B e antigen (HBeAg) positive and 62 (88.57%) were HBeAg negative. Materials and methods The study was done in 70 chronic HBV-infected patients. The present study was undertaken to detect HBcAg in chronic hepatitis B (CHB) patients and to assess the efficacy of detection system by indirect immunofluorescence (IIF) and indirect immunoperoxidase (IIP).
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The identification of hepatitis B core antigen (HBcAg) in hepatocytes may have variable clinical significance. Precore and core promoter mutants resemble inactive carrier status. Tabassum, Shahina Al-Mahtab, Mamun Nessa, Afzalun Jahan, Munira Shamim Kabir, Chowdhury Mohammad Kamal, Mohammad Cesar Aguilar, Julioīackground Hepatitis B virus (HBV) infection has many faces. Hepatitis B Core Antigen in Hepatocytes of Chronic Hepatitis B: Comparison between Indirect Immunofluorescence and Immunoperoxidase Method